The present study aimed to find differences among EBV-positive samples, HPV-positive samples, HPV/EBV-coinfected samples, and control samples concerning the expression levels of EBV and HPV genes, HPV integration physical status, and expression levels of some inflammatory, tumor-suppressor, antiapoptotic, and anoikis-related mediators, including IL-17, IL-11, IL-6, IL-1, IL-8, TNF-α, NF-κB, VEGF, TGF-β, ROS, RNS, Rb, p53, survivin, Bcl-2, CD44, Twist, E-cadherin, N-cadherin, PTPN13, and Slug. This evidence concerns the gene TNF and neoplasm.