While the inhibition of PKC-β or Nox2 improved BBB integrity and function in a similar manner to therapeutic hypothermia in hyperglycaemic settings, the combination of therapeutic hypothermia with either inhibitor almost completely neutralised the deleterious effect of hyperglycaemia on barrier integrity or function, proving a synergism in effect (Fig. 1). This evidence concerns the gene PRKCB and Hyperglycemia.