For example, lung weight-to-tibia length ratio as a measure of congestive heart failure increased after 2 weeks of T4 treatment, two death events occurred, and on the molecular level, this was reflected in increased cardiomyocyte apoptosis, mTOR activation, and increased expression of heart failure markers Anp and Bnp in hearts of T4-treated TAC mice. Here, NPPB is linked to congestive heart failure.