FOXD3 and nasopharyngeal carcinoma: To elucidate the effect of FOXD3-AS1 in NPC cell lines, we transfected SUNE1 and HONE1 cells with shRNAs of FOXD3-AS1 (shF-1 and shF-2) and shRNA-vector (shCtrl) transiently to silence FOXD3-AS1 (Figure 2A).