In order to determine the role of SOX2-OT in the malignant progression of NSCLC, SOX2-OT was overexpressed in NCI-H460 cells which had relatively low expression of SOX2-OT, while SOX2-OT was silenced in A549 cells which had relatively high expression of SOX2-OT. The gene discussed is SOX2; the disease is non-small cell lung carcinoma.