IECs are pretreated with the IBD drugs Tofacitinib (Tofa), Methylprednisolone (MetPred), and Mercaptopurine (6-MP) for 1 h, followed by culture with the cytokine cocktail (IFNγ, TNFα, and IL-1β) for 3 h and collection of cells at T2 and T13 to assay the expression of CEACAMs and IL-8 secretion. The gene discussed is TNF; the disease is inflammatory bowel disease.