Therefore, in this study, we intended to investigate the mRNA levels of five negative TLR regulators (SOCS1, TOLLIP, SIGIRR, TNFAIP3, and MyD88s) in PBMC and TNF-α secreting cells from patients of MDD, compared to the healthy controls and after antidepressant treatment. The gene discussed is TNFAIP3; the disease is major depressive disorder.