Similarly, the AO/EB staining revealed the apoptotic nuclear changes in human colon carcinoma cells (HCT-116) induced by methanolic extract of leaves and fruits of Ligustrum vulgare L [46].Terpinen-4-ol induced nuclear condensation of Molt-4 leukemic cells was evident with annexin-V-FLUOS staining [47]. This evidence concerns the gene ANXA5 and colon carcinoma.