IL10 and neoplasm: During our experiments, even though the incorporation of IL-10 in CAR cassette didn’t inhibit the survival of CAR-T cells nor cause excessive proliferation of tumor cells, the increased proportion of Treg during CAR-T cell culture in vitro (Fig. 6g) and in the microenvironment in vivo was observed (Fig. 8e), which may dampen the therapeutic effect and clinical outcome [40].