Due to these bi-directional behavioral effects, with antidepressant-like phenotypes after Nsun2 ablation in postnatal forebrain but increased depression-like behaviors in mice expressing a Nsun2 transgene selectively in adult PFC neurons, we next sought to determine if we could recapitulate the anti-depressant-like effect with a PFC-specific neuronal Nsun2 ablation in adulthood. This evidence concerns the gene NSUN2 and depressive symptom measurement.