Lastly, we observed an overexpression of FRYL at mRNA and protein levels in CRPC C4-2B and small cell prostate adenocarcinoma PC-3 cells when compared to androgen-sensitive LNCaP cells, indicating a putative role of FRYL in aggressive PCa (Figure 4C and Supplementary Figure 2). This evidence concerns the gene FRYL and posterior cortical atrophy.