This is evident as targeting CerS6 to reduce C16:0 ceramides, resulted in the prevention of diet-induced obesity and glucose intolerance (14, 16), whilst the ablation of CerS2 reduced C22:0 and C24:0 ceramides, increased C16:0 ceramides and contributed to spontaneous hepatocellular carcinoma development (18). Here, CERS6 is linked to obesity due to melanocortin 4 receptor deficiency.