When an indication of a possible hemoglobinopathy was found in the family history, microcytic hypochromic parameters or abnormal separation on IEF, HPLC, CE, or Sanger sequencing was applied to detect variants in the alpha- and beta-globin genes; however, six cases of rare anemia disorders were reported, which were diagnosed by NGS as the first-tier method (Bharadwaj et al., 2020; Rizzuto et al., 2021). This evidence concerns the gene HBB and hemoglobinopathy.