After 14-week HF/HS diet feeding, gene expression analysis of iWAT of the AAV-Notum treated mice revealed significantly decreased mRNA levels of Wnt target genes, WNT1-inducible-signaling pathway protein 1 (Wisp1)21 and WNT1-inducible-signaling pathway protein 2 (Wisp2)21, and increased expression of thermogenic/beige adipocyte-enriched genes, including cell death inducing DFFA like effector a (Cidea), purinergic receptor P2X 5 (P2rx5), Pgc1a, PR/SET domain 16 (Prdm16), and Ucp1 compared to those of the AAV-GFP mice (Fig. 4a). This evidence concerns the gene CCN4 and hydrops fetalis.