Moreover, several ECM-related genes, which levels changed in TM cells upon GLIS1 knockdown or overexpression, have previously been implicated in elevated IOP and glaucoma, including members of the collagen I, IV, and VI families, LTBP2, a regulator of TGFβ signaling and ECM deposition28,67. Here, TGFB1 is linked to glaucoma.