The enhanced phosphorylation of NFκB in CRC cells after palmitic acid treatment (Fig. 4D, E) suggests that the activation of the TLR4 signaling pathway is, at least in part, due to the enhanced TLR4, MyD88, and TIRAP expressions in the cancer cells but not a direct binding of palmitic acid to the TLR4 protein. This evidence concerns the gene TIRAP and cancer.