iTRAQ (isobaric tags for relative and absolute quantitation)-based proteomics with the tumor tissues also showed that knockout of TLR4 changed the metabolism of CRC (Fig. 6B–D) and also regulated other metabolic enzymes (column KO-HFD/HFD in Supplementary Table 4A) that the changes of many of these enzymes were not due to the dietary intervention (KO-HFD/KO-CD column in Supplementary Table 4A). This evidence concerns the gene TLR4 and neoplasm.