Interestingly, our data also showed that knockout of TLR4 abolished the HFD-enhanced β-catenin expressions in the tumor tissues (Supplementary Table 4B and Fig. 6G), suggesting that the energy homeostasis regulated by β-catenin [30] in CRC under HFD conditions is in relation to TLR4 activity. Here, TLR4 is linked to neoplasm.