Similar to two-dimensional PCa cell line culture, we did not observe enzalutamide-mediated changes to 6PGD mRNA expression in the PDE model over a time frame of 48 hr under conditions that caused significant repression of the well-characterised AR target genes KLK2 and KLK3 (Figure 1G). This evidence concerns the gene ALDH7A1 and posterior cortical atrophy.