To close this gap in knowledge, we performed migration experiments with ZO1 KO (single KO) and ZO1/2 double knockdown (dKD) Madin‐Darby canine kidney (MDCK II) cell lines as well as co‐cultures comprising dKD and wildtype (WT) cells in a 1:1 ratio accompanied by mechanical measurements and various imaging techniques. Here, TJP1 is linked to diabetic kidney disease.