1. PG (Emodin 80O-β-glucopyranoside) can reduce tumor growth and invasion in osteosarcoma mouse models by inducing pyroptosis (Tian B et al.);2. Dioscin can induce GSDME-mediated pyroptosis in osteosarcoma cells (Ding Q et al.);3. MiRNA-181a is abnormally elevated in osteosarcoma tissues and cells and the down-regulation of miRNA-181a can induce NLRP3-mediated pyroptosis (B.-G. TIAN et al.);4. The expression of GSDMD is relatively high in osteosarcoma tissues compared to normal (Rongjin Lin et al.). This evidence concerns the gene GSDMD and osteosarcoma.