MDSCs were analyzed in both tumor and spleen from CD45+CD11b+ populations gated from CD45+ live cells (Supplementary Figure 2B), and the granulocytic (CD11b+Ly6G+Ly6Clow) and monocytic (CD11b+Ly6G−Ly6C+) subpopulations were analyzed for the five treatment groups (Figures 5A, B). The gene discussed is ITGAM; the disease is neoplasm.