Gene ontology and pathway enrichment analysis revealed a significant association between TBX20 and important cancer-related pathways; downregulation was associated with cell cycle (P = 5.3 × 10−08) and double-strand break repair via homologous recombination (P = 9.2 × 10−07) in TBX20-deletion KYSE510s (Fig. 5g, Supplementary Table 6). Here, TBX20 is linked to cancer.