CD4 and neoplasm: First, we injected 1 × 106 THP-1-CD1c cells i.v. into NSG mice that, after tumor engraftment confirmed by their serum luciferase levels, were randomized into three groups, and sub-lethally irradiated, followed 24 h later by the transfer i.v. of 1 × 107 DN4.99 TCR-T cells (70% CD8+/30% CD4+), the same number of T cells (70% CD8+/30% CD4+) from the same donor undergoing parallel activation and expansion but no TCR transduction, or vehicle only (Fig. 7a).