Although previous results have suggested that ARRB1 can promote the phosphorylation of p65 in vivo and that phosphorylation of p65 promotes HCC cell proliferation through the GSK3β/mTOR pathway, it remains unknown whether the p65/GSK3β/mTOR signalling pathway is ARRB1 dependent in DEN-induced hepatocellular carcinogenesis. Here, GSK3B is linked to hepatocellular carcinoma.