We also observed that pretreatment with TNF-α (40 ng/ml) for 6 h promoted the expression of ARRB1 and p-p65 in HCC cell lines and found that p-Akt, p-GSK3β, p-mTOR and PCNA were markedly increased, consistent with the data obtained from WT mice. This evidence concerns the gene ARRB1 and hepatocellular carcinoma.