The expressions of human CEC-related markers (CD29, CD105, CD49e, CD166 and vimentin) were detected by FCM; likewise, the expression of Na+/K+-ATPase, ZO-1, CDH2, Col8a2 and SLC4A4 was detected by ICC and RT-PCR. The gene discussed is ALCAM; the disease is intrahepatic cholangiocarcinoma.