Consistent with the results from RNA‐seq profiling of the in vivo model, GSE41568 and SRR2089755 analysis, and IHC staining showed elevated PU.1 expression in clinical CRC liver metastases compared to primary CRC (Figure5A–C), which was further validated by Western blot of aforementioned pairs of primary CRC and liver metastases tissue samples and patient‐derived organoids (Table S2,S4, Supporting Information) (Figure 5D,E). The gene discussed is SPI1; the disease is colorectal carcinoma.