In addition, to confirm that HK2 can regulate EMT in CRC, we performed IHC staining to detect TJP1, E‐cadherin, vimentin and Twist1 expression in the tumour tissues of CRC patient samples (Figure 1E), and the immunohistochemical staining of these proteins exhibited different degrees of positivity (Figure S1C). The gene discussed is TWIST1; the disease is colorectal carcinoma.