Immunohistochemical staining was used to determine the expression levels of RUNX1, ARP2/3, TSP1 (Fig. 5b, d), and TGFβ1 (Supplementary Fig. 7) in tumor sections, which we found lower levels of their expression in RUNX1-knockdown specimens than their control counterparts. Here, TGFB1 is linked to neoplasm.