Our immunofluorescence staining demonstrated upregulation of both proteins in CRC cells upon exposure to recombinant TGFβ1, while the effect of TGFβ1 was impaired in the presence of RUNX1 inhibitor (Ro5-3335) (Supplementary Fig. 6a and 6b). This evidence concerns the gene TGFB1 and colorectal carcinoma.