Here, we investigated the transcriptomes of Drosophila heads from the same lines driving G4C2x36 expression by D42-GAL4: (i) healthy control flies expressing 3 G4C2 repeats and a luciferase-RNAi control (G4C2x3_C-RNAi); (ii) C9ORF72-ALS model expressing 36 G4C2 repeats and the RNAi control (G4C2x36_C-RNAi); (iii) C9ORF72-ALS-neuroprotected flies expressing 36 G4C2 repeats and the disrupted SRSF1 allele (G4C2x36_SRSF1-RNAi). Here, LGALS4 is linked to amyotrophic lateral sclerosis.