The CM1132 kit targeted 162 genes (Additional file 1: Table S1) known to cause deafness (include the genes’ whole exome, Coding + flanking intronic regions (10 bp) + deep intronic variants included in HGMD database), while the M113 kit (include the whole mitochondrial gene) contained two pathogenic hot-spot variants (NC_01292.1: m.1494C>T; NC_01292.1: m.1555A>G in MT-RNR1) that cause deafness. Here, MT-RNR1 is linked to deafness.