For instance, melatonin suppressed the renin-angiotensin system in the kidney in 5/6Nx models [52], inhibited fibroblast-myofibroblast trans-differentiation during renal fibrosis in unilateral ureteral obstruction mice [53], and mitigated oxidative stress in diabetic nephropathy rats [54]. This evidence concerns the gene REN and renal fibrosis.