In addition, the results of our study demonstrated that the mRNA levels of Wnt1, Wnt5a, Wnt7a, and Wnt9a were lower in osteoporosis-derived EVs than in non-osteoporosis-derived EVs (Figure 4), while DKK mRNA was up-regulated in osteoporotic individuals compared to non-osteoporotic individuals. The gene discussed is WNT5A; the disease is osteoporosis.