PRMT5 and infection: Furthermore, our results showed that HBV de novo infection (or transfected with HBV-expressing pCH-9/3091 plasmid) obviously reduced the levels of Rme2sy and H4R3me2s in HepG2-NTCP and HepG2 cells (Figure 1D-E; Table S2; Figure S1C-D), suggesting that HBV replication attenuates the methylase activity of PRMT5 in vitro.