In squamous cell carcinoma, CAFs were observed to increase the stiffness of ECM, which activated the Yes-related protein (YAP)/transcriptional co-activator PDZ binding motif pathway and induced the expression of YAP downstream genes, glutaminase (GLS1), LDHA, and SLC1A3, thereby activating the glycolytic and glutamine metabolic pathways within the tumor cells. The gene discussed is GLS; the disease is neoplasm.