AFF4 and leukemia: Similarly, compound 1 dose-dependently reduced the nuclear levels of H3K79-Me1/2 and ENL in MLL-AF4 driven MV4;11 leukemia cells (Figure 3C), while it did not cause significantly decreased AFF4 at 10 μM, presumably due to direct recruitment of AFF4 by MLL-AF4 through heterodimerization.