Our additional findings in the novel tank test, showing that EtOH consistent with prior reports11,61 reduces anxiety-like behaviors under novel conditions only at the 0.5% but not 0.1% concentration while increasing Hcrt neurons only at the 0.1% concentration, suggests that the reduced anxiety at the 0.5% dose in adult females may be attributed to a mechanism other than Hcrt, such as a reduced release of cortisol that is also observed at higher EtOH concentrations in zebrafish62. The gene discussed is HCRT; the disease is Anxiety.