The reduction in tumor growth in 3G8-fOva immunized mice expressing FcγRIIIB and FcγRIIA correlated with the generation of Ova-specific CD8+ effector T cells and higher CD8+ effector to Treg cell ratios in the spleens of these mice versus other groups (Fig. 7b, Supplementary Fig. 7b). In summary, a single immunization of GM-CSF treated mice with 3G8-fOva attenuates melanoma growth and results in the generation of antigen-specific CD8+ effector T cells. The gene discussed is CD8A; the disease is neoplasm.