To assess the kinetics of neutrophil to nAPC conversion after IC treatment and potential changes in nuclear morphology that accompany this change, we imaged mature peripheral blood neutrophils obtained from mice with YFP “knocked-in” to the CD11c locus (CD11c-YFP), labeled with a nuclear dye and treated with SLE-IC (in the absence of GM-CSF). Here, CSF2 is linked to systemic lupus erythematosus.