There are three methods commonly used to model AD in cerebral organoids: using Aftin-5 (an Aβ42 agonist) to induce Aβ42 peptide production in cerebral organoids, which resembles the SAD human brain [64]; producing cerebral organoids from iPSCs derived from FAD patients [65, 66]; and converting APOE3 to APOE4 in SAD patient-derived iPSCs to create differentiated SAD cerebral organoids [67]. Here, APOE is linked to Alzheimer disease.