Following the lentiviral knockdown of WDR43 in DLD-1 cells, WB was used to detect the expression of several proteins associated with tumor cell proliferation and migration, including N-cadherin, E-cadherin, Slug, Snail, VIM, MYC, fibronectin 1 and matrix metalloproteinase-2, and classic signaling pathway proteins and their phosphorylated forms, including β-catenin, p-β-catenin, extracellular signal-regulated kinase (ERK)1/2, p-ERK1/2, P-38, p-P38, protein kinase B (Akt), p-Akt, nuclear factor-κB (NF-κB) p65, p-NF-κB p65, mechanistic target of rapamycin (mTOR) and p-mTOR. This evidence concerns the gene MMP2 and neoplasm.