In the current study, we presumed that Fe3O4 MNPs may deliver BIRC5 and AS-ODN to tumor cells, which may be applied in radiotherapy, and set out to perform a series of experiments to investigate the molecular mechanism underlying small interfering RNA against BIRC5 (siBIRC5) in lung adenocarcinoma, hoping to discover some promising strategy to increase the radiosensitivity. This evidence concerns the gene BIRC5 and lung adenocarcinoma.