RSAD2 and Rotavirus infection: To investigate whether viperin was induced during rotavirus infection, we used western blot analysis to evaluate viperin expression in the lysates of HT-29 cells, either mock-infected or infected with RV-SA11 (MOI 3) at different hours post-infection (0 hpi, 3 hpi, 6 hpi, 9 hpi and 12 hpi).