To investigate the role of PKR in the IFN-I response triggered by a NW arenavirus infection, we measured the levels of IFN-β mRNA in control and PKR knockout human lung epithelial A549 cells (A549/PKR KO) infected with TCRV and JUNV-Candid#1. This evidence concerns the gene EIF2AK2 and Arenaviridae infectious disease.