To investigate whether TGF-β1 production is more in the vemurafenib-resistant melanoma cells than BRAFV600E mutated melanoma cell lines, two BRAFV600E mutated melanoma cell lines A375 and SK-MEL-28 and their vemurafenib-resistant cells lines were used in ELISA assay to compare the amount of TGF-β1 release from the same number of cells. This evidence concerns the gene TGFB1 and melanoma.