PML and infection: Analogous experiments using human fibroblasts depleted for PML (HFF/shPML) as well as control cells (HFF/shControl) likewise revealed an enhanced initiation of RCMV gene expression (Figs 8E and S5) as well as RCMV replication (Fig 8F) in absence of PML, thus further substantiating the role of PML-NBs as a barrier for cross-species infection.