After an application interval of 24 h, significantly higher tumor/background ratios could be achieved with [89Zr]Zr-PSMA-DFO than with [68Ga]Ga-PSMA-11 and [18F]F-JK-PSMA-7 at 2 h and 4 h after application. The gene discussed is FOLH1; the disease is neoplasm.