Tumor/liver ratios (F(2,10) = 101.7; p < 0.0001) of [89Zr]Zr-PSMA-DFO were significantly higher compared to [68Ga]Ga-PSMA-11 and [18F]F-JK-PSMA-7 at 2 h, and significantly higher compared to [18F]F-JK-PSMA-7 at 4 h. Here, FOLH1 is linked to neoplasm.