Figure 4(D) further indicated that miR-146b overexpression reduced the levels of total STAT3 in the ovarian cancer cell lines. We then examined the level of phosphorylated STAT3 protein and found that tyrosine phosphorylation and serine phosphorylation of STAT3 were both significantly decreased after miR-146b overexpression in the ovarian cancer cell line (Figure 4(E)). The gene discussed is STAT3; the disease is ovarian cancer.