In this inquiry, the protein expression of c‐Caspase‐1 in breast cancer cells under DOX treatment was lightly reduced within 24 hours, while the expression patterns of GSDMD‐F, GSDMD‐C IL‐1β and NLRP3 hardly changed (Figure 2A, B, C and D). The gene discussed is NLRP3; the disease is breast cancer.