Future studies that characterize CFS EVs from individuals should assess whether this pattern persists at the individual sample level and whether it is: a result of the EV isolation method, characteristic of CFS EVs, and/or whether this finding of low CD81 expression is characteristic of CFS EVs from patients with asthma in comparison to healthy controls. The gene discussed is CD81; the disease is myalgic encephalomeyelitis/chronic fatigue syndrome.