ADs upregulated the top pathways including MT fission, protein localization, membrane fusion, organelle fusion, neurodegeneration with brain iron accumulation (NBIA) subtype pathway, insertion of tail-anchored proteins, calcium ion transmembrane transport, vesicle fusion, Fragile X syndrome, MT localization, response to inorganic substance, regulation of NIK/NF-kB signaling, bile acid metabolic process, cholesterol metabolism, lung fibrosis, and protein targeting. This evidence concerns the gene NFKB1 and fragile X syndrome.