To study the role of AMPK–Pdlim5 pathway in the development of atherosclerosis, ApoE−/− animals were divided randomly into streptozocin-induced diabetes mellitus together with or without metformin, Pdlim5 phosphomimetic mutant that carried adenovirus (Pdlim5 S177D) or Pdlim5 unphosphorylatable mutant that carried adenovirus (Pdlim5 S177A). This evidence concerns the gene PRKAA2 and atherosclerosis.