To explore the clinical significance of these six genes, pancancer analysis in BLCA (Figure 3A) and 23 other tumors (Supplementary Figure 1) was performed, and the results revealed that the expression of TSHZ3, ZFHX4, ZEB2, MEIS1, and ISL1 was significantly lower than that in normal tissues, while the expression of HOXC4 was higher than that in normal tissues, especially in BLCA, breast invasive carcinoma (BRCA), prostate adenocarcinoma (PRAD), and head and neck squamous cell carcinoma (HNSC). The gene discussed is ISL1; the disease is invasive breast carcinoma.